Objective To investigate the effect of IGHG1 gene silencing on migration, proliferation and apoptosis of human bladder cancer EJ cells. Methods Bladder cancer cell line EJ cells were transfected with LVIGHG1 -RNAi and taken as experimental group (shIGHG1 -EJ cells). Negative scrambled lentiviral vector was used to transfect EJ cells, which were treated as negative control group (NC-EJ cells). EJ cells without transfection were LVused to be blank control group (EJ cells). The expressions of IgG γ, caspase-3 and cleaved caspase-3 were detected by Western blot and IGHG1 mRNA expression by qRT-PCR. CCK-8 was used to detect the proliferation of the three groups of cells. Flow cytometry (FCM) was used to detect the cell apoptosis. Wound healing assay was used to investigate the change in migration of shIGHG1 -EJ cells. Results The transfection rate of the shIGHG1 -EJ cells was (76.667 ± 0.042)%, and the transfection rate of the NC-EJ cells was (75.333 ± 0.055)%. The expressions of IGHG1 mRNA and IgG γ of the shIGHG1 -EJ group were obviously lower than those of the control groups (P < 0.05), which showed that IGHG1 gene silencing was successful. CCK-8 revealed that IGHG1 gene silencing significantly decreased the proliferation of EJ cells (P < 0.05). FCM and Western blot demonstrated that IGHG1 gene silencing promoted the apoptosis of EJ cells (P < 0.05), and the cleaved caspase-3 fragment appeared at 17 kda. Wound healing assay showed that cell migration was inhibited by IGHG1 gene silencing (P < 0.05). However, NC-EJ cells had no meaningful difference from EJ cells (P > 0.05). Conclusions IGHG1 gene silencing can observably inhibit the migration and proliferation of human bladder cancer EJ cells. It can also promote cell apoptosis.