Objective To observe the effect of acupuncture on liver and renal antioxidant capacity in arsenic poisoning mice. Methods Sixty health Kunming ICR mice were randomly divided into normal group (15 mice) and arsenic poisoning group (45 mice). After establishment of arsenic poisoning liver and kidney injury model, the mice of the arsenic poisoning group were randomly divided into model group, acupuncture group and curcumin group with 15 in each group. In the acupuncture group, the mice were given needling treatment of Zusanli Point, Neiting Point and Tianshu Point. The mice of the model group were not treated, those in the curcumin group were given curcumin intervention (200 mg/kg). After 30 days the mice were sacrificed, the content of malondialdehyde (MDA), SOD, GSH and GSH-PX in the renal and liver tissues were measured by enzyme-linked immunosorbent assay, and were compared with the normal group. Results Compared with the normal group, the content of MDA and GSH and the activity of SOD, and GSH-PX in the liver and kidney tissues were not significantly different in the acupuncture group (P > 0.05); the content of MDA in the liver and kidney tissues was significantly higher (P < 0.05), the GSH content and the activity of GSH-PX and SOD were significantly lower (P < 0.05) in the model group. Compared with the model group, the content of MDA was significantly lower, the content of GSH and the activity of GSH-PX and SOD were significantly higher in the acupuncture group (P < 0.05). Conclusions Acupuncture may reduce the content of MDA and increase the content of GSH and the activity of GSH-PX and SOD in the liver and renal tissues of mice, alleviate arsenic poisoning damage to the liver and kidneys.