Objective To investigate the expression and clinical significance of tumor markers, pituitary tumor transforming gene (PTTG), vascular endothelial growth factor receptor -3 (VEGFR-3) and hemodynamic indexes in patients with benign or malignant cervical lesions. Methods Totally 98 patients with pathologically confirmed cervical lesions were admitted into our hospital from March 2012 to February 2016 and were involved in this study. Cervical tissuesand blood samples of all patients were collected. The expressions of PPTG and VEGFR-3 were determined by Immunohistochemistry. Levels of serum squamous cell carcinoma antigen (SCC), carbohydrate antigen 19-9 (CA199), carbohydrate antigen 125 (CA125), microvessel density (MVD), peak systolic velocity (PSV), end diastolic velocity (EDV) and vascular resistance index (RI) were identified. Clinical significance of above-mentioned indexes in patients with benign or malignant lesions were analyzed. Results Levels of SCC, CA199 and CA125 in patients with cervical intraepithelial neoplasia (CIN) were increased compared with those in patients with chronic cervicitis(P < 0.05),while those were downregulated when compared with patients with invasive cervical carcinoma (P < 0.05). The positive expression rates of PTTG and VEGFR-3 in patients with cervical intraepithelial neoplasia (CIN) were increased compared with those in patients with chronic cervicitis (P < 0.05), while those were downregulated when compared with patients with invasive cervical carcinoma (P < 0.05).MVD, PSV and EDV in patients with cervical intraepithelial neoplasia (CIN) were upregulated comparedwith those in patients with chronic cervicitis (P < 0.05), while those were decreased when compared with patients with invasive cervical carcinoma (P < 0.05). However, RI decreased in patients with CIN when compared with patients with chronic cervicitis (P < 0.05), while that was increased when compared with patients with invasive cervical carcinoma. Conclusion Patients with invasive cervical carcinoma experience increased levels of serum cancer markers, positive rates of PTTG and VEGFR, MVD, fast blood flow and decreased vascular resistance, which may be potential prognostic biomarkers.