Objective To investigate the effects of plateau environment on fetal growth and development using two-dimensional ultrasonogrphy. Methods A total of 103 normal pregnant women in the Affiliated Hospital of Qinghai University (2,300 m above sea level) from June to December 2016 were enrolled into the observation group. And 481 cases of normal pregnant women undergoing prenatal examination in the same period in the Civil Aviation School of Clinical Medicine, Peking University (44 m above sea level) were enrolled as the control group. Biparietal diameter (BPD), head circumference (HC), abdominal circumference (AC) and femur length (FL) of fetuses in the two groups were measured by two-dimensional ultrasonography. Results BPD, HC, AC and FL of the fetuses were compared between the observation group and the control group. Since these variables were in non-normal distribution, non-parametric test was used. None of the measured parameters was significantly different between the two groups (P > 0.05). Conclusions The environment at 2,300 m above sea level does not have significant effect on the growth and development of normal pregnant fetuses.