Objective To study the expression of MicroRNA-577 (miR-577) in human lung squamous cell carcinoma and its effect for NCI-H520 cell invasion. Methods Totally 60 patients with lung squamous cell carcinoma and paired non-tumor tissues, who were collected during January 2013 to December 2016 were involved in this study. Expression of miR-577 was determined by qRT-PCR. MiR-577 mimics were used to over-express miR-577 in NCI-H520 cells. Cell invasion was identified by Transwell assay. The expression of β-catenin in NCI-H520 cells were detected by qRT-PCR and Western blotting. Results MiR-577 expression was decreased in lung squamous cell carcinoma tissues compared with normal tissue (P?