Objective To discuss the value to evaluate voice function in the group by comparing and analyzing the differences of voice range profile and voice fatigue test in occupational voice users who were with or without the vocal cord lesion. Methods According to inclusion and exclusion criteria, 112 occupational voice users were randomly divided into normal group and pathological group. There were no obvious hoarseness, dysphonia and vocal lesions (such as chronic vocal cord inflammation, vocal cord nodules, vocal cord polyps, etc) in the normal group. The pathological group had hoarseness, dysphonia or vocal cords. The voice range profile and voice fatigue test were separately examined to quantify the voice functions of the two groups. Results Compared with the normal group, the average frequency of the fundamental frequency perturbation increased (P < 0.05), the average minimum sound intensity increased (P < 0.05), and the failure rate of voice fatigue test increased (P < 0.05), but the average of the maximum sound intensity decreased (P < 0.05), the average of the maximum frequency decreased (P < 0.05), the average of the minimum frequency decreased (P =0.003), the average of maximum phonation time decreased (P < 0.05), dysphonia severity index decreased (P < 0.05) in pathological group. Conclusions The voice range profile and voice fatigue test are capable to evaluate the voice function and voice fatigue in occupational voice users, and can be used as important measures to evaluate the voice quality.