Objective To evaluate effect of adipose stem cell gel (SVF-gel) in breast augmentation with autologous fat transplantation. Method A total of 60 patients for breast augmentation from January 2017 to January 2019 were enrolled, and 20 cases of which adopted SVF-gel autologous fat transplantation in observation group, and other 40 patients received prosthetic breast augmentation in control group. Values of chest circumference and chest eminence and stable retention rate, complications and patients’satisfaction rate were compared. Results The values of chest circumference, chest eminence and stable retention rates after two weeks, one month, three months and six months in observation group were all significantly more than in control group (P < 0.05). The complications rate in the two groups was no difference (P > 0.05), while patients’ satisfaction rate in observation group was higher (P < 0.05). Conclusion Autologous SVF-gel breast augmentation is simple and safe, which can effectively improve effect of breast augmentation, increase long-term breast stability and improve patients’ satisfaction.