Objective To investigate the changes of liver tissue metabolites induced by carbon tetrachloride (CCl4) in mice after acute liver injury by gas chromatography-mass spectrometry (GC-MS), and to screen related metabolic pathways. Method A model of acute liver injury induced by intraperitoneal injection of 0.1% CCl4 (V/V) olive oil was established. The liver tissue samples of the blank control group and the model group were pretreated and derivatized to perform GC-MS based metabolism. Online data analysis using Metabo Analyst 3.0 was used to observe differential metabolites in the two groups and analyze relevant metabolic pathways. Result A total of 33 possible endogenous substances were identified by GC-MS analysis. 14 substances were identified by the map. The partial least squares method was used to distinguish the two groups of samples clearly and analyzed possible metabolic pathways by marker metabolites. Conclusion GC-MS metabolomics techniques can be used to evaluate animal models of acute liver injury in mice.