Objective To investigate the effect of lncRNA HSALNT0000015 on the biological behavior of colorectal cancer cells. Methods Quantitative real-time PCR (qRT-PCR) was used to detect the expression level of lncRNA HSALNT0000015 in 80 samples of colorectal cancer tissue and 15 adjacent tissues. And in colorectal cancer cell lines Lovo, SW480, HT-29, HCT116, and normal human mammary epithelium cell line HIEC. The HCT116 cells were divided into negative control (NC) group, negative shRNA-Lenti (vector group) or Lenti-sh-HSALNT0000015 (sh-HSALNT0000015 group). The cell proliferation ability was determined by MTT assay experiment; migration and invasion ability were measured by transwell assay; the transcription factor Slug, epithelial marker E-cadherin and interstitial marker vimentin were dectected by western blotting. Results The expression of HSALNT0000015 in the colorectal cancer tissues was significantly higher than that in the adjacent tissues (P?