Objective To investigate the effect of proliferation and invasion on hyperthermia combinated with cisplatin in human laryngeal carcinoma Hep-2 cells and whether the two treatments have an interaction. Methods The Hep-2 cells were randomly divided into 4 groups: control group (37℃), hyperthermia group (43℃), cisplatin group and hyperthermia combined with cisplatin group. The effects of hyperthermia combined with cisplatin on proliferation, migration and invasion of Hep-2 cells were determined by MTT assay, Wound-healing assay and transwell invasion assay. Results Four groups of cells were administrated with MTT. The OD value at 490 nm were (1.24?±?0.13), (0.99?±?0.11), (0.75?±?0.07) and (0.31?±?0.07) respectively. Factorial analysis showed that OD values at different temperatures were statistically significant (P?