Objective To investigate the ultrasonographic features of four molecular subtypes of breast cancer. Method From January 2014 to April 2020, 66 cases of breast cancer were diagnosed by color Doppler ultrasound and confirmed by surgery and pathology in our hospital. According to immunohistochemical results, breast cancer were divided into four molecular subtypes, i.e. Luminal A type (n = 13), Luminal B type (n = 29), HER- 2 over-expression type (n = 11), and triple-negative breast cancer (n = 13). The ultrasonographic features of four molecular subtypes of breast cancer were retrospectively analyzed, including the shape, spiculated margin, D/W ratio, fine calcification, posterior echo attenuation, blood flow, tumor sizes, lymph node metastasis, and histopathological grade. Results There was no statistically significant difference in the tumor sizes and histopathological grade among them (both P > 0.05); in the lymph node metastasis, there was a statistically significant difference (P < 0.05); ultrasonographic features (shape, spiculated margin, D/W ratio, fine calcification, posterior echo attenuation, blood flow) had statistically significant difference among four subtypes (all P < 0.05). Triple-negative breast cancer were statistically significant difference from those of Luminal A type (shape), Luminal B type (shape, D/W ratio, posterior echo attenuation), HER-2 over-expression type (fine calcification) (all P < 0.008). There was a significant difference between Luminal B and HER-2 over-expression type in fine calcification (P < 0.008). Conclusion Four molecular subtypes of breast cancer have certain ultrasonographic features. Clinicians can predict the four molecular subtypes of breast cancer according to the ultrasonographic features, which could provide more basises for the clinical treatment and diagnosis.