Objective To evaluate the correlation between myocardial motion from apical four-chamber view and that from short-axis view in normal rats via velocity vector imaging ( VVI ). Methods We selected 18 SD ( Sprague-Dawley ) rats at 10 weeks of age. The VVI was used to measure the longitudinal peak systolic velocity (Vs), peak diastolic velocity (Vd), peak strain (SR), peak systolic strain rate (SRs) and peak diastolic strain rate (SRd) of the left ventricle from the apical four-chamber view and the lateral and posteroseptal myocardium at mitral valve level from the parasternal short-axis view, and the correlation of myocardial motion between the two sections was analyzed. Results The Vd of lateral myocardium at mitral valve level from the parasternal short-axis view positively correlated to that of the basal segment of lateral wall of the left ventricle from the apical four-chamber view (r = 0.566, P = 0.014). The Vd, SRs and SRd of lateral myocardium at mitral valve level from the parasternal short-axis view positively correlated to those of the middle segment of lateral wall of the left ventricle from the apical four-chamber view (r = 0.521, 0.540, 0.592，respectively; P = 0.027, 0.021, 0.010, respectively). The Vd and SRs of posteroseptal myocardium at mitral valve level from the parasternal short-axis view positively correlated to those of the basal segment of posterior septum of the left ventricle from the apical four-chamber view (r = 0.614, 0.627, respectively; P = 0.007, 0.005, respectively). All the five indicators of posteroseptal myocardium at mitral valve level from the parasternal short-axis view positively correlated those of the middle segment of posterior septum of the left ventricle from the apical four-chamber view (r = 0.536, 0.937, 0.530, 0.639 and 0.621, respectively; P = 0.022, 0.000, 0.024, 0.004 and 0.006, respectively). Conclusion VVI technology can be used to evaluate the myocardial walls motion of left ventricle from the apical four-chamber view in rats.