Abstract: Objective To explore the application value of color Doppler ultrasonography in the prenatal screening of fetal malformations. Methods Totally 1,387 pregnant women (a total of 1,402 fetuses) who were detected by prenatal ultrasonography in our hospital from September 2014 to December 2015 were retrospectively analyzed. All the women had 1 or more times of color Doppler ultrasound examination and routine examination of the basic situation of the fetuses, then the images of each section were saved and the measured, the data were recorded. The detection results of fetal malformations were observed and compared with the clinical diagnosis after delivery or induced labor, and the application value of prenatal color Doppler ultrasonic diagnostic apparatus was analyzed. Results In the 1,402 fetuses, 46 were confirmed to have fetal malformations after delivery or induction of labor, and the malformation rate was 3.28% (46/1,402). Prenatal ultrasound screening found 41 cases with abnormal fetuses, and the detection rate of malformations was 2.92% (41/1,402); 4 cases had missed diagnosis and 1 case had misdiagnosis. Conclusions The application of color Doppler ultrasonography in prenatal screening of fetal malformations has a high detection rate, which can be used as a routine prenatal examination.