Objective To observe the effect of curcumol combined with fluorouracil (FU) on subcutaneous transplantation tumor tissues of gastric cancer cells in nude mice. Methods The implantation tumor model was built by subcutaneous inoculation of human gastric cancer cell line BGC823 in nude mice. The tumor-bearing mice were randomly divided into control group, curcumol group, FU group and combination group (curcumol combined with FU). Data of tumor volume and weight of each group were collected. Expressions of PCNA and P27 genes in tumors of the 4 groups were detected with qPCR and immunohistochemistry. Results The tumor volume was significantly smaller and the tumor weight was significantly lower in the 3 experimental groups than in the control group (P < 0.05). Compared with the curcumol group, the tumor volume was significantly smaller  and the tumor weight was significantly lower in the combined group (P < 0.05). The volume and weight of the tumors had no significant diffe-rences between the FU group and the combined group (P > 0.05). The expression of the PCNA in the experimental groups was significantly lower than that in the control group (P < 0.05). Compared with the curcumol group and the FU group, the expression of PCNA in tumor tissues of the combined group significantly decreased (P < 0.05). The expression of the P27 in the 3 experimental groups was significantly higher than that in the control group (P < 0.05). Compared with the curcumol group, the expression of P27 in the combined group significantly increased (P < 0.05). There was no significant difference in P27 mRNA of tumor tissues between the combined group and the FU group (P > 0.05). The positive percentages of p27 protein in the 3 experiment groups were significantly higher than that in the control group (P < 0.05). Compared with the curcumol group and the fluorouracil group, the positive percentages of PCNA protein in the combined group was significantly higher (P < 0.05). Conclusions Curcumol combined with FU could inhibit the growth of  subcutaneous transplantation tumor of human gastric cancer cells in nude mice, which might be related to regulation of PCNA and P27 genes.