Objective To study the influences of Isoflurane on learning and memory of APPswe/PS1AE9 double transgenic mice of Alzheimer disease (AD). Methods Transgenic mice were divided into Tg-iso group and Tg-con group, wild-type mice were divided into Wt-iso group and Wt-con group. At the age of seven months, the Tg-iso group and the Wt-iso group were exposed to 1.1% Isoflurane for 2 hours per day for 5 days. Meanwhile, the mice in the Tg-con group and the Wt-con group inhaled oxygen/nitrogen (FiO2 = 30%). Learning and memory abilities were tested 48 hours and 5 months after Isoflurane exposure using the Morris Water Maze and Y maze, respectively. Results At the age of seven months, mean latency in the Wt-iso group decreased in a place navigation test compared to the Wt-con group (P = 0.013); compared to the Tg-con group, the mean latency decreased in the Tg-iso group (P = 0.043); there was no significant statistical difference in the target quadrant time or distance percentage in probe trials among the groups (P = 0.392). At the age of twelve months, compared to the Tg-con group, the discrimination errors of the Wt-con and Tg-iso groups decreased (P = 0.044 and 0.020). Conclusions Repeatedlow-dose Isoflurane anaesthesia does not increase the dysfunction of learning and memory of APPswe/PS1AE9 double transgenic mice and wild type mice, to some extent, can even decrease dysfunction of learning and memory in APPswe/PS1AE9 double transgenic mice and wild type mice.