Objective To study the change situation of serum and cerebrospinal fluid insulin and interleukin related indexes of patients with acute lymphatic leukemia. Methods From October 2011 to August 2015, 57 patients with acute lymphatic leukemia in our hospital were selected as the observation group, and 57 healthy persons of the same age with the health examination at the same time were selected as the control group.  Then the serum and cerebrospinal fluid insulin and interleukin related indexes of two groups were respectively detected and compared. The serum and cerebrospinal fluid detection results of the observation group with different FAB types were compared too. Results The serum and cerebrospinal fluid IL-2 of the observation group were both lower than those of the control group, and other serum and cerebrospinal fluid interleukin related indexes were all higher than those of the control group. Serum IGF-1 and IGFBP-3 levels were all lower than those of the control group, serum IGF-2 level was higher than that of control group. Cerebrospinal fluid insulin related indexes were all higher than those of control group, and the serum and cerebrospinal fluid detection results of observation group with different FAB types had obvious differences, and the differences were all significant (P < 0.05）.Conclusions The serum and cerebrospinal fluid insulin and interleukin related indexes of patients with acute lymphatic leukemia shows obviously abnormal state and the detection results′differences of patients with different FAB types are obvious too, so those detection indexes all have higher detection value for the patients.