Objective To investigate the disinfection and deformation effect on the gypsum models prepared with effective chlorine solution and gypsum powder blended in the vacuum mixer. Methods The positive serum specimen of hepatitis B antigen (HBeAg and HBsAg HBV DNA ≥ 107 copies/ml) was selected and  intentionally polluted the oral alginate impression, vacuum mixer was used to mix up tap water, effective chlorine solution (2 500 mg/L) and gypsum powder respectively, then filled them into the models which were designed to be the control model group and the observation model group respectively. The optical density value/critical value of HBV was compared. The patients' oral alginate impression were collected and applied as models, after the tap water, effective chlorine solution (500 mg/L) and gypsum powder were mixed up by a vacuum mixer, the mixture was filled into the models. These models were designed to be the control group and the observation group respectively. Bacterial colonies on the surface of the models between the two groups were analyzed. The solidation time of the gypsum models made of gypsum powde and water and models made of the powder and different concentrations of chlorine solution were compared. The models made by the standard laboratory impressions were filled with blended 500 mg/L and 2500 mg/L effective chlorine solutions with tap water and gypsum powder respectively. The precision and hardness of the models before and after disinfection were analyzed. Results The density value/critical value of the hepatitis B viruses were (1.11 ± 0.11) and (0.60 ± 0.03) respectively; the counting results of bacterial colonies on the surface of gypsum models of the control group and observation group were respectively (12.11 ± 0.11) and (2.60 ± 0.73); the optical density value / critical value and the counting results of the bacterial colonies of the observation group were obviously lower than those of the control group, and the inter-group comparison differences were statistical meanings significant(P < 0.001). However, the inter-group comparison was differed of setting time precision and hardness of the models before and after disinfection with the effective chlorine solution had no statistical difference(P > 0.05). Conclusions Application of gypsum models prepared with effective chlorine solution and gypsum powder blended in the vacuum mixer can effectively kill the bacteria, eradicate the hepatitis B viruses, and reduce the risk of cross infection without affecting deformation of the models. This method of disinfection is convenient, practical and easy to be spread.