Objective To investigate whether protein of hepatitis B virus induced the expression of IL-32. Methods HepG2 was mediated transfected by Lipofectamine 2000 TM. After 48 hours, the expressions of IL-32 mRNA and protein in HepG2 cells were detected by RT-PCR and western blot respectively. Results RT-PCR result showed expressions of IL-32 mRNA in HepG2 cells transfected with HBV were 2.3 times higher than the cells transfected with empty vector (non-transfected) . Western blot result showed that, compared with the IL-32 protein in untransfected HepG2 cells, expression of IL-32 protein in transfected pIRES2-HBV-EGFP HepG2 cells was increased, and the difference was statistically significant (P < 0.05). Conclusion The expression of IL-32 mRNA, IL-32 protein in HepG2 cells is promoted by HBV.