Ⅰ,Ⅱ期宫颈癌组织HIF-1α与COX-2蛋白共表达对预后的影响及机制
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李欣,E-mail:lixinyc5189@163.com

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宜昌市医疗卫生科研项目(No:A14301-31)


Influence of the coexpression of HIF-1α and COX-2 on prognosis  of patients with cervical cancer in stageⅠand stageⅡ and possible functional mechanism
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    摘要:

    目的  探讨Ⅰ,Ⅱ期宫颈癌组织中HIF-1α与COX-2蛋白的共表达对预后的影响及可能的机制。方法  免疫组织化学法分析71例宫颈癌标本的HIF-1α和COX-2的共表达情况;RT-PCR法检测标本中HIF-1α mRNA和COX-2 mRNA的表达水平。χ2检验分析不同表达组患者1、2和3年存活率的差异;采用KM生存分析法了解不同表达组患者的存活时间的差异;采用相关分析了解蛋白及mRNA表达水平的相关性。结果  HIF-1α和COX-2共表达组患者的3年存活率低于非共表达组(P <0.05);HIF-1α和COX-2共表达组患者的存活时间低于非共表达组(P <0.05);在Ⅱ期患者中,HIF-1α的蛋白水平与COX-2 mRNA的水平正相关(rs  =0.594,P <0.05)。结论  在Ⅰ,Ⅱ期宫颈癌中,HIF-1α和COX-2共表达组预后较差;HIF-1α蛋白可能正向调控COX-2 mRNA的表达。

    Abstract:

    Objective To investigate the influence of the coexpression of HIF-1α and COX-2 on the prognosis of patients with cervical cancer in stageⅠ and stage Ⅱ and the possible functional mechanism. Methods The coexpressions of HIF-1α and COX-2 were detected in 71 cases with cervical cancer by immunohistochemical. The levels of HIF-1α mRNA and COX-2 mRNA were detected by RT-PCR. Chi-squared test was used to analyze the differences of survival rate from one year to three year in different groups; KM survival analysis was used for the survival time in different groups; correlation analysis was used to analyze the correlation between protein and mRNA level. Results The survival rate of three year in the group of coexpression of HIF-1α and COX-2 was significantly lower than that in the non-coexpression group (P < 0.05). The survival time in the group of coexpression of HIF-1α and COX-2 was significantly shorter than that in the non-coexpression group (P < 0.05). There was a positive correlation between the HIF-1α protein level and the COX-2 mRNA level in the patients with cervical cancer in stageⅡ (rs= 0.594, P < 0.05). Conclusions The prognosis is poorer in the group of coexpression of HIF-1α and COX-2 of patients with cervical cancer in stage Ⅰ and stage Ⅱ than the non-coexpression group. The HIF-1αprotein may upregulate the level of COX-2 mRNA.

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罗爱华,丰大利,付娟,李欣.Ⅰ,Ⅱ期宫颈癌组织HIF-1α与COX-2蛋白共表达对预后的影响及机制[J].中国现代医学杂志,2016,(16):67-71

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  • 收稿日期:2016-04-14
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  • 在线发布日期: 2016-08-31
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