Objective To analyze the relationship of the adverse reactions of fundus fluorescein angiography (FFA) and indocyanine angiography (ICGA) with the patients' age, gender and disease types.Methods We selected 31,475 patients with anaphylaxis test being negative and undergoing FFA and/or ICGA in our hospital from January 2000 to January 2019 as the study subjects, and the incidence, time to onset and severity of adverse reactions, examination items, gender, age, and types of eye diseases were recorded.Results A total of 4,679 patients developed adverse reactions, with 4,367 mild cases (13.87%), 277 moderate cases (0.90%) and 22 severe cases (0.07%), and 95.72% of the adverse reactions occurred within 5 minutes. Specifically, 4,125 patients undergoing FFA alone had adverse reactions, with 3,813 mild cases (15.34%), 277 moderate cases (1.11%), 22 severe cases (0.09%) and 13 undetermined cases. There were differences in the age, gender and disease composition between patients with and without adverse reactions after FFA alone (P < 0.05). In contrast, 872 patients were examined by ICGA alone, and only 73 patients (8.4%) had mild adverse reactions, with differences in the age and disease composition (P < 0.05) but no difference in gender composition between patients with and without adverse reactions (P > 0.05). There were still 5,749 cases examined by both FFA and ICGA, and 481 of them (8.4%) had mild adverse reactions. The age and disease composition (P < 0.05) rather than the gender composition (P > 0.05) were different between patients with and without adverse reactions in these cases.Conclusions Anaphylaxis test can not completely predict the occurrence of adverse reactions. Young patients are more likely to develop adverse reactions after FFA and ICGA, and different diseases contribute to different risks of adverse reactions. We need to pay more attention to young patients and diseases that are more susceptible to adverse reactions within 1 h after angiography to ensure safety.