Objective To investigate the application of steel wire suspension in gynecological gasless single-port access laparoscopic surgery.Methods The clinical data of 60 patients with uterine lesions and 60 patients with adnexal lesions treated in Yongkang First People's Hospital from March 1, 2020 to October 28, 2020 were analyzed retrospectively. Among them, 30 patients with uterine lesions and 30 patients with adnexal lesions received gasless single-port access laparoscopic surgery with steel wire suspension (group A), while the remaining 60 patients received traditional laparoscopic surgery (group B). The surgery process, complications and patient satisfaction of the two groups were compared and analyzed.Results Compared with group B, the ooperative duration, intraoperative blood loss, length of hospital stay, the recovery time of intestinal function, Visual Analogue Scale (VAS) score at 24 h following the operation, length of hospital stay, and the recovery time of intestinal function were shorter, intraoperative blood loss was less, and VAS score at 24 h following the operation was lower in group A (P < 0.05). The overall incidence of postoperative complications in group A (5.00%) was lower than that in group B (21.67%) (P < 0.05). Besides, the patient satisfaction in group A (93.33%) was higher than that in the group B (80.005%) (P < 0.05).Conclusions Applying steel wire suspension to gasless single-port access laparoscopic surgery is conducive to reducing the surgical trauma, promoting postoperative recovery, relieving the pain, lowering the incidence of complications and improving patient satisfaction. Thus, it can be practiced in the treatment of gynecological diseases.