Osteoarthritis (OA) is the most common form of joint disease, and is the leading cause of pain and disability by affecting knee, hip, wrist, spine and other joints. However, the pathogenesis of osteoarthritis is still unclear. PI3K/Akt/mTOR signaling pathway regulates cell growth, differentiation, and migration, as well as angiogenesis and metabolism, and is also essential for apoptosis and autophagy. Recent studies have found that PI3K/Akt/mTOR signaling pathway is involved in the occurrence and development of osteoarthritis. This paper reviews the research progress on the roles of PI3K/Akt/mTOR signaling pathway in osteoarthritis, aiming to provide novel ideas for the prevention and treatment of osteoarthritis.