Objective To explore the application effect of computer-assisted navigation and 3D printing technology in the reduction and titanium plate fixation of zygomatic arch fractures.Methods A total of 82 patients with zygomatic arch fractures admitted to our hospital from April 2018 to April 2021 were selected and randomly divided into the study group and the control group, with 41 cases in each group. The control group was given conventional reduction and titanium plate fixation with the aid of 3D printing technology, and the computer-assisted navigation was additionally applied in the study group on the basis of the control group. The operative duration, intraoperative blood loss, fracture healing time, the outcome of reduction, molecules associated with bone healing, the density of the callus, the accuracy of reduction, and postoperative complications were compared between the two groups.Results There was no difference in the operative duration and intraoperative blood loss between the two groups (P > 0.05). The fracture healing time of the study group was shorter than that of the control group (P < 0.05). No difference in the excellent and good rate of the fracture reduction was revealed between the two groups (P > 0.05). The differences of the levels of bone morphogenetic protein 2 (BMP-2) and transforming growth factor-β₁ (TGF-β₁) before and after the operation in the study group were higher than those in the control group (P < 0.05). The density of the callus at 1 month and 3 months after the operation was higher in the study group compared with the control group (P < 0.05). The difference between the width of the bilateral zygomatic arches and that between the protrusion of bilateral zygomatic bones were lower in the study group relative to the control group 3 months after the operation (P < 0.05). There was no difference in the overall incidence of postoperative complications between the two groups (P > 0.05).Conclusions The application of 3D printing technology could achieve satisfying clinical outcomes with or without computer-assisted navigation in the reduction and titanium plate fixation of zygomatic arch fractures. However, the aid of the computer-assisted navigation may improve the accuracy of the reduction and promote the fracture healing.